Abstract:

A method was set up based on microsatellites for distinction between pork and beef. According to the inter-species specificity of the microsatellites, some loci of bovine and porcine were selected from Genebank, named SignⅠand Sign II, and the primers were designed for each microsatellite according to the flanking sequence. The DNA samples were extracted from 48 individuals of cattle and pig for PCR reactions, which products were used to Electrophoresis. The results show that the target strips were observed which were 379bp and 585bp separatively. These strips can identify which sample from cattle or pig. This method is accurate, specific, quick and simple, and the result is easily judged.