Abstract:

To screen the strains with high production of laccase from A. Auricula so that the properties and gene expression of A. Auricula laccase as well as its structures and functions can be studied. 10 strains were brought from Henan Agricultural Academy of Science and Henan Institution of Biology，respectively. All strains grown on PDA plate containing 0.04% guaiacol were selected according to their diameters and shades of coloration circle. When further screening was taken in submerged fermentation，Dimao-1 showed highest level of laccase activeity at 363.85U/L after cultured in shaken flasks for 11d. Dimao-1 was potential strains of A. auticula for further research in laccase gene clone and expression. Three kinds of laccased were identified by the colorness development of zymogram stained with guaiacol after Native polyacrylamide electrophoresis.