Abstract:

The HA gene of SIV amplified by PCR, was subcloned into eukaryotic expressing vectors pcDNA3.1.we examined the antibody response to DNA vaccine and inactivated virus and the protection against H3N2 SIV challenge using mouse model. Mice were immunized with different immune formation. Three weeks after the second immunization, mice were challenged with a high dose of H3N2 SIV. The ability of protection against virus challenge in mice was evaluated by induction of serum antibody response, the lung virus titers of mice. The results demonstrate the immune response induced by different immune formation in mouse model has been proved to be able to protect against the challenge with H3N2 SIV.