Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2009, Vol. 25 ›› Issue (21): 54-58.

Special Issue: 油料作物 园艺

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The research on the molecular identification and coincidence rate on CMS hybrid seeds purity of Brassica napus

  

  • Received:2009-08-07 Revised:2009-08-22 Online:2009-11-05 Published:2009-11-05

Abstract:

The RAPD standard map of CMS hybrids Qinyou 7 and Qin zayou1 that were popularized in practice was constructed according to plenty of primers selections and times of replication, and the hybrid seeds purity of those two commercial were detected. The research on correlation between the samples that represented the seed population and field planted population was analyzed, which revealed that the correlation coefficient was over 97% between the molecular detection result and field performance, this results indicated that the method for analyzing the seeds purity of rapeseed hybrids was reliable. This method was successively used in 5 years in practice on hundreds of samples, ten thousands of single plant and more that twenty thousand kg hybrid seeds.