Abstract:

In order to investigate the protecting role of heat shock proteins in the cross-adaptation to temperature stresses induced by cold acclimation (CA) or heat acclimation (HA). Leaves from two-years-old young grape (Vitis vinifera L. cv. Jingxiu) plants were used as experimental materials. The subcellular localizations of HSP70 within mesophyll cells were observed using immuno-gold labeling electron microscopic technique. The results showed that CA pretreatment could enhance the expression level of HSP70 during heat stress. Compared to non-acclimated plants, the density of HSP70 gold particles was obviously increased in the nucleus and chloroplast of the CA leaves, especially 3 h after heat stress. Similar to CA, HA pretreatment can protect the expression of HSP70 during cold stresses, and the density of HSP70 gold particles remains high level than that in control plants. These data offered the cytological evidence of HSP70 was contributed to the cross-adaptation to different temperature stresses induced by CA-, or HA-pretreatment in grape plants.