Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (4): 300-303.

Special Issue: 生物技术 园艺

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Study on Comparison of Methods for Pitaya Total DNA Extraction

  

  • Received:2009-08-28 Revised:2009-12-13 Online:2010-02-20 Published:2010-02-20

Abstract:

Abstract: The tissues of Pitaya richly contain polysaccharide and other subsequent metabolizing substance, so it is difficult to extract DNA effectively. The study compares different methods for Pitaya DNA extraction on tender stem of Pitaya.. Then the purified DNA was detected through Eppendorf Biophotomete. The researches indicated that modified CTAB and micro-CTAB extraction methods were both suitable for extracting DNA of Pitaya, especially the latter. DNA purity gained by those two methods was available to the further molecular biology study.