Abstract:

Abstract: With the tender stem segments with partial root of Ophiopogon Japonicus (L.f.)Ker-Gawl as the explants, the induction of sterile plantlet, formation of re-generated plantlet, differentitation and growth of adventitious root were studied. The results showed that disinfecting with 75% alcohol for 20s and 0.1% HgCl2 for 10 min had the best disinfection effect. The effect of bud-inducing and multiplication was better on the medium MS+6-BA2.0mg/L+NAA0.2mg/L，and the inducing rate of asepsis plantlet was 80%, the inducing rate of clumpy buds was 100％．The average of clumpy buds was 6.24 on the propagation of fourth and could perfectly meet the need for rapid multiplication. The inducing rate of adventitious root with the best medium 1/2MS+NAA0.5mg/L was 100%. When transplanted in the farm after two weeks, the survival rate of plantlets was 100%, and the plantlets were vigorous.