Abstract:

The plant EST (expressed sequence tags) program is one of the most important parts which compose the plant genome program. The expansion of EST data base is foundation of development of EST markers. In this paper, the development of five EST markers (EST-SSR, EST-PCR, EST-SNP, EST-AFLP, and EST-RFLP) and their application on comparative genomics, phylogeny and function studies of gene. Some problem exit in the development of EST markers is also discussed.