Abstract:

Abstract：From the method of the foreign DNA introduced to Arabidopsis thaliana via flowers dip, we used stigma smear method to transfer T-DNA into watermelon and research T-DNA molecular marker. The results showed the ideal tranform specie is ZXG01078 with the highest fruit setting rate and the most deviant seedlings. The best concentration of the watermelon seeds treated by kanamycin is 500～700mg/L with differences among the species; the best concentration of the watermelon leaf treated by kanamycin is 4000～8000mg/L, and the best position is spire leaf or youngleaf with no difference among the species. The steadily variation appearing of no pointing and twinborn seedling indicated the normal growth interfered by foreign DNA in the progeny.