Welcome to Chinese Agricultural Science Bulletin, Jul. 30, 2025

Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (4): 200-204.

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Studies on Browning Control in Tissue Culture of Acer×freemanii ‘Autumn Blaze’

  

  • Received:2009-09-09 Revised:2009-09-24 Online:2010-02-20 Published:2010-02-20

Abstract:

The measures including different collecting time, adding antibrowning reagents and culturing in darkness were carried out in terms of severe browning phenomenon in the process of Acer×freemanii ‘Autumn Blaze’ tissue culture. The effects of three browning control measures were studied in the paper. The results were as follows: The browning rate increased with months changed; The antibrowning effects with AC and PVP showed extremely significant difference with contrast to that of Vc and Na2S2O3. The browning control effects of PVP with concentration of 2.0g/L was optimal; The browning rate presented higt-low-high tendency with the darkness time increased. Measures of collecting explants in April and May, inoculating on MS adding 2.0 g/L PVP, culturing under the light directly could control browning effectively.