Abstract:

The conditions of isolation and purification of protoplast from somatic embryogenic cell suspension of Allium sativum L. were studied. The results showed that the optimum condition was obtained when using 2.0% Cellulase Onozuka R-10,0.2% Pectolgase,0.55 mol/L mannitol, 5mM MES for 5h. High yield and viability protoplasts were obtained when purified by centrifugation at 1000r/min for 5min.