Abstract:

A kind of white rot related fruition bodies on the trunk of Fraxinus mandshurica was diagnosed as Inonotus hispidus by the characters of morphology. Specific polymerase chain reaction (PCR) of the internal transcribed spacer (ITS) region was carried out successfully from DNA extracted from fruiting bodies. The length of the PCR product is 500bp. Phylogenetic analysis of the ITS region for 11 species of Inonotus (10 was from NCBI) was done. The phenogram inferred from the alignment suggested that I. hispidus and I. radiatus were evolutionarily closely related to one another in this study. I. circinatus and I. tomentosus were evolutionarily closely related to one another; I. cuticularis and I. rickii were evolutionarily closely related to one another; I. dryophilus and I. rheades were evolutionarily closely related to one another. I. circinatus, I. tomentosus, I. hispidus and I. radiatus might had the same ancestor; I. cuticularis, I. rickii, I. obliquus and I. glomeratus might had the same ancestor; I. dryophilus, I. rheades and I. tamaricis might had the same ancestor.