Abstract:

The epidermal and sub- epidermal thin cell-layer of flue-cured tobacco (Nicotiana tabacum L) tender stem in additional appropriate hormone combinations of the MS culture medium can take one-step shoot formation. MS+NAA0.1～0.3 mg/l +IBA0.1 mg/l +6-BA1.5～2.5 mg/l+3%Sucrose culture medium is suitable for one-step shoot formation in thin cell layer cultures. BA concentrations below 2.5mg / l is conducive to nurturing strong seedling. 1/2MS+2% Sucrose +NAA0.1～0.2 mg/l culture medium is suitable for root induction.