Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (6): 47-50.

Special Issue: 园艺

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Establishment of regeneration system of cotyledon and hypocotyl in vitro of tomato

  

  • Received:2009-11-12 Revised:2009-12-02 Online:2010-03-20 Published:2010-03-20

Abstract:

Abstract: The cotyledon and hypocotyl from tomato asepsis seedlings were cultured on the medium with different 6-BA and NAA concentrations to study their effects on the callus induction and shoot differentiation. The result indicated that some light green callus could be induced and adventitious buds could be differentiated in all kinds of medium, but there were obvious different in different medium. The optimum medium for tomato callus formation and bud differentiation respectively were MS+6-BA2 mg/L+IAA0.2 mg/L and MS+6-BA2 mg/L+I AA 0.02mg/L. The best medium for inducing tomato root was MS + IAA 0.2 mg/L.