Abstract:

Abstract:【OBJECTIVE】Synthesis of CAP-BSA,CAP-OVA, polyclonal antibody against chloramphenicol, and comparison of titer of anti-sera from CAP-BSA,CAP-OVA.【METHOD】Bovine serum albumin (BSA) and Ovalbumin (OVA) were used as protein carries respectively to couple with chloramphenicol（CAP）by diazotization method, then compared the immune ability of the two synthetic antigens. The two synthesized antigens CAP-BSA and CAP-OVA were mixed with the same amount of adjuvant and then immunized rabbits. After the fourth immunization, the rabbits were blooded from heart, and then separated blood sera; lastly we issued indirect competitive ELISA analysis using the two antigens as coatingen respectively.【RESULTS】Enzyme linked immuno-sorbent assay (ELISA) indicates the existence of specific antibodies in the anti-sera. The titer of anti-sera for CAP-BSA and CAP-OVA is 1:8100. The detection limit for CAP was 9ng/mL.【CONCLUSION】After comparison of the results comprehensively, we believed that using CAP-OVA as immunogen and CAP-BSA as coatingen is a good experimental program. This experimental program can be used for preparation of polyclonal antibody against chloramphenicol and production of chloramphenicol ELISA Kit.