Abstract:

In this study, the primers of sequence was designed on tomato 45S rDNA , tomato genomic DNA as a template was amplified by PCR out of ITS sequences (internal translation spacer) , the cloned sequence is based on two wild tomato species and three cultivated species for molecular Identification and molecular analysis of genetic relationship. The results showed that: (1) five of the test materials are a small variation of ITS sequences, GC content was high up to57.0% ~ 65.4%. (2)using different clustering analysis showed that three cultivars tested materials have a closed genetic relationship, while the wild species of currant tomato (Solanum pimpinellifolium )are in different branches of taxa, indicated that their kinship with other species is relatively distant, at the same time the biological characteristics of Solanum pimpinellifolium significantly are different from other species.