Abstract:

The effect of sucrose and Vc on H2O2 accumulation and root growth inhibition of Arabidopsis were investigated in hydroponics. We found that Cr inhibits root elongation with increasing concentrations of Cr. Meristematic regions and the elongation zones were greatly shortened, suggesting cell division and cell elongation are all affected. Plant roots died of Cr overdose. DAB staining analysis showed that hydrogen peroxide (H2O2) accumulated in root tips when the seedlings were treated with Cr. Furthermore, we found that inclusion of sucrose (Su) and vitamin C (Vc) can dramatically increase the tolerance of the seedlings to Cr treatment. Taken together, the results suggest that inhibition of root growth induced by Cr is likely mediated by ROS production.