Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (6): 286-288.

Special Issue: 资源与环境

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Screening of Primers in Genetic Diversity Analysis of Pyrus Germplasm by AFLP

  

  • Received:2009-11-02 Revised:2009-06-15 Online:2010-03-20 Published:2010-03-20

Abstract:

In this study, 10 primer combinations selected from 64 ones were identified to be high polymorphic. The amounts of bands were 23-55.A total of 371 DNA bands were amplified, including 306 polymorphic bands. The10 primer combinations were high polymorphic primers, which could be applied widely on Pyrus L.By AFLP technique. AFLP technique was applied on germplasm genetic diversity.It was abundant in Pyrus germplasm resources in China. It might be helpful for studying Pyrus germplasmic resources by screening of primers on AFLP.