Abstract:

Using wheat bran and corncob as main material, the solid state fermentate medium of Trichoderma pseudokoningii which produce the most spores was screened. Single factor experiments were studied on the proportion of wheat bran and corncob, water content, and the additives. The optimized medium is composed of: wheat bran: corncob=8:2, water content 55~60%, KNO30.1%, calcium sulfate1%，sucrose1%. Shallow tray fermentation experiment was performed, the results show that under the conditions of 28℃, inoculum size of 10%, and the optimized medium, 470g dried mycelium and spores were obtained from 1000g dried raw material. One gram of dried fermentation products contains 1.34×109 spores. The research provides a theoretical basis for mass production of Trichoderma pseudokoningii mycelium and spores agents.