Abstract:

with unique fragrances and a variety of health functions, Lentinula endodos is one of main cultivated mushroom in China. However, in some regions of China in recent years have witnessed varying degrees of mushroom virus disease,causing severe influences in yield and quality. Through experiences it showed that the virus is widely existed in Lentinula edodes.Three Lentinula edodes strains were selected to eliminate virus by picking up hypha tips(less than 0.2mm in length) . The results showed that:some strains with one band of dsRNA (11kb) left in Lentinula edodes strains were got, which called incomplete eliminated strain. only one strain was got with no dsRNA in it ,which called virus-free strain. the virus- elimination rate is very low by this method. The growth rate of the three kinds of strains in different pH were measured, it showed that the virus-free strain grew faster than virus-infected strain in pH 8 condition. cellulase, xylanase, laccase and polyphenol oxidase activity of the three kinds of strains were mensurated ,the result showed that the enzyme activity of Laccase and Polyphenol oxidase in virus-free strain were higher than that of virus-infected strain.Through the cultivation experiments ,the fruit body of virus-free strain is hypertrophy than others, and with a stronger anti-pollution ability.