Abstract:

Abstract：The study was conducted to cryopreserve canine semen for optimizing cryopreservation medium. The different concentration of sugar (Glucose Fructose), different balance times, different fumigation times were selected systematically in the cryopreservation medium. The results showed addition of 0.5% fructose was better than glucose at any concentration(P<0.05). 120 min balance time and 20 min liquid nitrogen fumigation time were excellent for motility of frozen sperm (P<0.05). In conclusion, the optimized procedures(0.5% fructose, 120 min balance time and 20 min ) were feasible and steady for freezing canine sperm. Key words：Canine, Sperm, Glucose, Fructose, Cryopreservation