Welcome to Chinese Agricultural Science Bulletin,
CASB

Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (10): 17-20.

Special Issue: 生物技术

• 23 • Previous Articles     Next Articles

Detecting the expression efficiency of the RNA replicon vaccines and DNA vaccine of HBV in BHK-21 cells

Li Li, Ren Linzhu, Zhang Ying, Yang Dong, Yan Sen, Li Xiaoping,   

  • Received:2009-12-10 Revised:2010-03-02 Online:2010-05-20 Published:2010-05-20

PDF (PC)

1017

Abstract:

Abstract: Construction of RNA replicon vaccine and DNA vaccine of HBV, then the 2 vectors were tranfected into BHK-21 cells and their expression efficiency was detected by ELISA with the kits used to detect HBsAg and preS2. Meantime, we found that the vectors based on pSFV can make BHK-21 cells dead (apoptosis). It is confirmed that the immune effect of RNA replicon vaccine is better than that of DNA vaccine. RNA vaccine is safer and more prospects.