Abstract:

Abstract: (Objective) This experiment was designed to study the effect of rumen hydrogenation by concentrate supplement; incubation time; microflora.(Method) Cattle in four treatments were fed full-fat soybean, baking soybean and healthy soybean.And these were treated respectively by protozoa , bacteria , fungi and gastric juice. (Result)The method of rumen fermentation in vitro was used to study the effect of various concentrate supplement, the rumen micro-organisms, incubation time to rumen hydrogenation. The results showed that: NH3-N was effected mainly by different materials and the interaction between Microflora and incubation time,the content of NH3-N was significantly higher in the full-fat soybean group than the soybean baking group,but had no significant difference with health soybean. The concentration of NH3-N in the fungi and Rumen fluid medium was increased fastest and it was at the maximum position all long. VFA concentration was effected mainly by the interaction between microflora and incubation time and little by different concentrate supplement. The concentration of propionate and butyrate were higher in the group of rumen liquid. Different material, incubation time and interaction between them had significantly effcet to the content of stearic acid.the remarkable influence to the concent of stearic acid was produced only in some time in rumen microflora. Concentration of linoleic acid(18:2) was impact only by different rumen microflora. Different material, incubation time and interaction in rumen microflora had significantly effect to the concentration of trans-oleic acid (18:1) and linolenic acid (18:3). (Conclusion) Fungi in the supplementary materials play an important role in fermentation, VFA was the result of interaction between different micro-organisms,adding full-fat expanded Soybean had a positive effect on the synthetic-CLA and its precursors.