Abstract:

The genetic diversity of 52 E. sibiricus accessions were evaluated by SSR markers. A total of 318 fragments were identified with 18 SSR primers sets, of which 204 (86.48% ) were polymorphic, and each primer generated 13.1 fragments, the polymorphism information content (PIC) per primer was 0.267~0.471, with an average of 0.35, and the marker index (MI) of SSR was 3.98. The genetic similarity (GS) coefficient of the tested accessions ranged from 0.622 to 0.895, with an average of 0.766. The Nei’s index of diversity (He) of the 52 accessions was 0.3286, and the Shannon’s index (Ho) was 0.4851. These results suggested that there was rich genetic diversity among the tested wild resources of E. sibiricus. 52 accessions could be divided into five main groups by cluster and principal component analysis, the accessions from the same region or with the same habitat type tends to were classified into the same group, indicating the geographical distribution of genetic diversity of E. sibiricus.