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Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (7): 36-39.

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Establishment of RAPD reaction system for Panax ginseng rhizosphere soil microbial community

  

  • Received:2009-12-11 Revised:2009-12-18 Online:2010-04-05 Published:2010-04-05

Abstract:

In this study, RAPD reaction system that fitted for genetic diversity study of Panax ginseng rhizosphere soil microbial community was established. Based on the optimized RAPD reaction system, 38 out of 200 RAPD primers having polymorphic were identified. Furthermore, genomic DNA extracted from soil samples were amplified by polymorphic RAPD primers identified. Results indicated that, the optimized RAPD reaction system was fitted for genetic diversity study of P. ginseng rhizosphere soil microbes.