Abstract:

Abstract:Zhongmiansuo 48 and its parents were amplified with 25 pairs of core primers.Out of them,16 paris were detected to be polymorphic between the two parents.14 pairs primers of the 16 pairs were codominant markers since they amplified the different fragments between the two parents and heterozygosis bands in F1.The other 2 pairs of primers generated the same bands between one of parents and F1,So they were dominant markers. Digital fingerprint of Zhongmiansuo 48 were constructed with the amplification result by changing binary system into decimal system.It provides a method for seed genuineness and purity detection of hybrids. In order to construct the correct, rapid, stable and practical fingerprint ,we suggest regulating the types and numbers of the molecule markers , standardizing the reagent quality of DNA,Taq et al. Uniforming conditions of PCR reaction, electrophoresis and Staining; offering standard varieties, standard DNA; standardizing the identification of electrophoresis bands and setting down ways of data coding