Abstract:

For researching transformation of Lycium barbarum. L ,the multigene expressed vector KCTB that constructed with four functional genes, GUS、BAR、KC1、CAF1 was introduced into Lycium barbarum. L mediated with agrobacterium tumefaciens. Analysis of transgenic plant by histochemical staining showed that the GUS was expressed predominately in leaves, and also lesser degree in roots. The result of PCR suggest that BAR、KC1、CAF1 was detected and also the KCTB was contained in genome of Lycium barbarum. L.