Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (9): 277-282.

Special Issue: 园艺

• 23 • Previous Articles     Next Articles

Population Dynamics of Endophytic Bacteria in Tomato Plant and Screening of Antagonistic Strains

Wang Meiqin1, Liu Huiping1, Han Jucai1, Lu Tao2   

  • Received:2009-12-25 Revised:2010-01-12 Online:2010-05-05 Published:2010-05-05

Abstract:

Abstract:In order to study distribution and population dynamics of endophytic bacteria in tomato plant, 374 endophytic bacteria strains of tomato plants were isolated and purified from 3 cultivated regions. By conventional methods, these strains were identified as 13 genus. Among them, Bacillus,Pseudomonas,Xanthomonas and Entenobact were the dominant genus.The endophytic bacteria were the most common in the tomato plants. Results indicated that number of endophytic bacteria ranged from 0.21x104 to 13.82x104 cfu.g -1fresh weight,but there were distinguish between the number and variety of endophytic bacteria in different tissue,different variety, different ages and different cultivated regions. The number of endophytic bacteria was the largest in roots, followed by in stems and leaves,and the number and variety of endophytic bacteria in Maturity plants were higher than in seedling plants. Of all isolates,32 strains were known to express antibiosis activities against Botrity cineane , and 25 strains were known to express antibiosis activities against Fulvia fulva,20 strains were determined in vitro antagonism to both of the pathogens,there have been 12 strong antagonistic strains against Botrity cineane,but only 1 against Fulvia fulva.. Bacillus sp.were the dominant genus.