Abstract:

Abstract：To find a simple,rapid and effective total DNA extraction method for Dracaena cambodiana Pierre ex Gagnepain,four methods, CTAB、SDS、SDS-CTAB、Improved CTAB method,were compared.The total DNA was detected by agarose electrophoresis,Spectrophotometer and ISSR amplification.The result showed that the A260/A280 of total DNA extracted by the Improved CTAB method ranged from 1.7 to 1.9 with high-purity,low levels of impurity and good integrity.ISSR fragments amplified with DNA template extracted by Improved CTAB method showed clear bands,and good polymorphic,stability and repetition.The Improved CTAB method was effective for total DNA extraction Dracaena cambodiana Pierre ex Gagnepain,which was suitable for PCR amplification and other molecular biology researches.