Abstract:

The aldehyde dehydrogenase (ALDH) was studied as target gene in this study, and the plant expression vector pBI-ALDH has been constructed.Tomato 03HN31 Cotyledon was transformed by A.rhizogenes R1000. We detected the transformed plants by multiple molecular biology methods (PCR, outhern blot and RT-PCR) and resistance-correlated physiological indices (relative electric conductivity and malondialdehyde content). Results showed that the ALDH gene had been integrated into the genome of tomato and expressed at transcriptional level. Moreover, the relative electronic conductivity and malondialdehyde (MDA) content showed different between transformed tomato plants and controls under stresses of drought, high-salt and hypothermia etc, which indicated that the damage of transformed plants plasma membrane was decreased the antioxidant capacity was increased under stress. The transformation ratio is 10.78%.