Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (11): 330-335.

Special Issue: 资源与环境 食用菌 食用菌 水产渔业

• 23 • Previous Articles     Next Articles

Establishment and preliminary application of scar marker in germplasm resource of Pleurotus abalones

Xiong Fang, Zheng Minjiang, Liu Xinrui, Xie Baogui   

  • Received:2010-01-13 Revised:2010-02-11 Online:2010-06-05 Published:2010-06-05

Abstract:

In order to solve the chaotic phenomena of naming Pleurotus abalonus variously in the market, this article mainly analyzes 16 Chinese regular Pleurotus abalonus cultivating bacterial strains by SRAP-PCR and RAPD-PCR augmentation, and then choose the special segments from electrophoretogram, transform the segment into SCAR mark, and further classify these 16 bacterial strains into 5 categories through the 4 pairs of SCAR particular primer, so as to settle the chaos of giving various names to the same kind or going to the contrary in the abalone mushroom market. This article not only provides the evidence for the foundation of the core classification of Pleurotus abalonus and the scientific record of the relevant resources, but also lays a foundation for the product standard rate establishment and for the standardized manufacture.