Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (12): 22-25.

Special Issue: 园艺

• 23 • Previous Articles     Next Articles

Screening Random Primers for RAPD analysis in Cauliflower Cultivars

Chen Yang1, Zhou Xianzhi1, Chen Sheng1, Wu Yufen1, Zhao Yijie2   

  • Received:2010-01-26 Revised:2010-02-22 Online:2010-06-20 Published:2010-06-20

Abstract:

【OBJECTIVE】To elucidate the genetic diversity and phylogenetic relationships of 37 cauliflower cultivars, provide a theoretical basis for cauliflower breeding, 80 random primers were carried out a preliminary screening with 7 among the cauliflower cultivars as amplification template. 【RESULTS】Among which 48 primers gave polymorphism, 25 primers gave monomorphic and 7 primers without amplified bands within the varieties. 【CONCLUSION】Stable, clear and polymorphic bands could be obtained with primers S11, S15, S16, S17, S18 and S65, the percentage of polymorphic bands ranging from 16.7% to 75.0%, and these primers appear to be useful for genetic diversity studies in cauliflower cultivars.