Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2005, Vol. 21 ›› Issue (2): 10-10.

Special Issue: 玉米 烟草种植与生产

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Application of SSR Markers in Maize Inbred Lines for Studying Their Genetic Relationship

Wang Fengge,, Zhao Jiuran, She Huadi, Chen Gang, Guo Jinglun, Dai Jingrui   

  • Online:2005-02-05 Published:2005-02-05

Abstract: A set of 38 elite maize (Zea mays L.) inbred lines, representative of various types of inbred lines with and without clear pedigree, were assayed with 62 pairs of SSR primers. Our main objectives were to (ⅰ) investigate the variation of the SSR primers in these materials, (ⅱ) assigning inbred lines to heteotic groups, (ⅲ) examine the usefulness of SSR markers in assaying genetic relationship of maize inbred lines. 238 alleles were found with average alleles 4.08,average polymorphism information content (PIC) 0.612, average marker indice (MI) 2.58 per locus. the UPGMA cluster analysis separated the 38 inbred lines into 7 major groups based on their SSR genotypic similarities, including six major temperate groups( Reid, Luda Red Cob, Tangsipingtou, LSC, P1,P2) and one tropic Suwan group,and the genetic relationship of inbred lines with complex genetic were determined by SSR markers.