Abstract: Abstract：The microspores of 5 genotypes of Brassica campestris L. ssp.chinensis and 1 genotype of Brassica campestris L. ssp.pekinensis were cultured in vitro using modified NLN medium (1 / 2 of inorganic major element) and the procedure of microspore culture of Brassica napus. The results showed that the embryo yield between genotypes was obviously different. The embryos regenerated from the microsores of 5 out of 6 genotypes with 83.33% of tested materials.The embryo yield of zhongqiqingkangre605 was the highest with 8.676 embryos per 10 buds.All embryos produced from the microspores of 5 genotypes developed into stronger plantlets on B5 medium added NAA and PP333. The embryogenesis capacity of microspores of 6 genotypes needs to be further studied.