Abstract: By measuring the root-knot nematode infection of cucumber within 48 hours after the endogenous plant hormone content changes, we studies the initial stage of infection between the endogenous plant hormone homeostasis, analysis the stress response and physiological mechanisms of cucumber root-knot nematode disease, in order to find the induction of plant disease resistance theoretical reference. The results show that when cucumber plant was infected by Meloidogyne spp., the height, the fresh weight of the above-ground part and the root were decreased significantly. By enzyme-linked immunosorbent assay（ELISA），we analyzes the changes of endogenous hormones content four hours a time till 48 hours after the inoculation. Results indicated that the content of Jasmonic acid (JAs) in the leaf of infected plant was increased to 820％ of that in the leaf of control plant during 16 hours after the inoculation。The contents of Salicylic acid (SA), Abscisic acid（ABA）and iPAs were also increased significantly after the inoculation, they all achieved their peak values 24 hours after the inoculation. While the content of IAA and gibberellin1+3(GA1+3) were lower than that in control plant except transitory increase during the 48 hours.