Abstract:

The identification method for the uninucleate stage of tomato microspore, and the best method to free microspore from anther are studied in this paper. Carmine acetate dyeing, Alcohol- Hydrochloride-Carmine acetate dyeing, DAPI staining were used to stain the cell nuclear of microspore in uninucleate stage. Neutral Red with pH value 6.8-8.0 was used to stain the vacuole. Squeezing method, squeezing centrifuge and Natural spill out were used to free the microspore from anther. Results show that Carmine acetate dyeing is not effective in coloring compared to Alcohol Hydrochloride-Carmine acetate dyeing. Clear coloring can also be obtained by DAPI staining and Neutral Red staining. However, these two methods are limited by high cost, testing time or the activity of microspore. Lowest in advance hulling rate can be reached by Natural spill out , compared to squeezing and squeezing centrifuge method. Therefore, Alcohol- Hydrochloride-Carmine acetate dyeing is the best identification method for the DEVELOPMENTAL STAGES of tomato microspore. And Natural spill out can be used to free the microspore, which can reduce the hulling rate and improve the activity.