Abstract: The spatial analysis functions of Geographic Information System (GIS) combined with the traditional zoning methods could get more precise results of agro-climatic division. GIS as a kind of technological means has been widely used in agro-climatic division, which provide a reliable basis for making a decision of agricultural production. Summing up the application progress on agro-climatic division based on GIS in China is discovered from four points such as climate factors fine-gridding, map digitizing, information services and “3S” technologies combination. Furthermore, some problems in the application are discussed and potential research directions are pointed out in order to provide some reference for researchers in the field. Some suggestions are as follows: (1) Many kinds of natural factors and social factors should be taken into account compositely when doing agro-climatic division; (2) The “bottom-up” of the region merging process by GIS needs to combine with the auto-merge and manual-merge more better; (3) Zoning indexes should be considered using the logical intersection operation; (4) Setting up the Web-based open platform for sharing the agro-climatic division of GIS; (5) GIS combine with GPS, satellite remote sensing data could comprehensive monitor the whole process of crop growth from a macro point of view, and have an important application value for directing agricultural production.