Abstract:

Abstract: There are multi-nucleus in hypal cells and no clamp-connections in morphology on Volvariella volvacea.That makes its difficulty to cross breeding.In this study,using SRAP method for screen out different genetic bands between single-spore strain 26 and strain 28,and optimizing temperature conditions of PCR primers designed on sequence of bands, finally ,we got four different SCAR genetic markers, which SCAR1 and SCAR4 belong to strain 26 specially , SCAR2 and SCAR3 belong to strain 28 specially. This four SCAR genetic markers have successful identified the hybrid nature of the strains 2628 and will be applied to genetic analysis of hybrid offspring. It reveals the nature of the genetic model of straw mushroom and provides an effective means.