Abstract:

The endophytic bacterial strain TS-5 from medicinal plants of Densefruit pittany, root-bark was tested for its inhibiting effect against Botrytis cinerea. The physiological and biochemical characters, antagonistic activity, classification and growth condition of TS-5 in vitro were measured. The results showed that the strain had antagonistic activity against the gray mold disease, the inhibition zone was 10mm , antagonistic rate on leaves was 33.72%. According to the morphology observation and physiological and bio-chemical reactions, the strain was identified as Bacillus subtilis. Tests showed that the optimum growth condition is 30℃ at pH 7 for 48 hour. The bacterial strain is aerobic.