Abstract: Abstract: Oriental tobacco bacterial leaf spot disease is a new bacteria l disease infected by Pseudomonas syringae pv. tabaci (Wolf et al. ) Young et al. and is the main disease of oriental tobacco. The oriental tobacco varieties were identified for their resistance to bacterial leaf spot disease, with the method of natural disease plot and manual inoculation. The results indicated that the incidence trends of different variety differed obviously. The variety Base kona Basma、Basma No. 14, Luoxiang No. 1, Sam sum pathogenesis time was ealier, and the plant’s condition did not turn worsen, the variety multi-leaf oriental tobacco was later and the plant’s condition was expanding speedly. At 21 days after inoculation, the leaf was dried up of variety Izmir NO. 14, Kokulu??zmir, Turker Tuka-3 (Turker Basma×Carabaglar), Luoming No. 2-1, multi-leaf oriental tobacco. Of all the other 23 varieties, there were mid-susceptible, 43.48% mid-resistant and 30.43% high-resistant. Variety Basma No. 1, Carabaglar, Canik, Luoxiang No.a, Sam sum, Basma No. 14 and Base kona Basma was high resistant to bacterial leaf spot disease.