Abstract:

【OBJECTIVE】This paper is to clearify the in vitro clearance ability of -OH, DPPH- and NO2- with the mycelial polysaccharides from Pleurotus ostreatus, P. nebrodensis and P. eryngii;【METHOD】Mycelial polysaccharides were extracted by hot water and purified by Sevag method. The anti-oxidation activity was determined by polyfunctional microplate spectrophotometer. The data were analyzed by DPS V7.05 software;【RESULTS】. The clearance rates of -OH, DPPH- and NO2- were 43.51％-98.09％, 5.98％-51.96％ and 10.22％-26.75％ respectively in the polysaccharide concentration range of 200-2000 μg/mL. The EC50 values of DPPH- clearance rate for B10, PL7 and P89 are 1.98, 2.74 and 3.28mg/mL respectively. The EC50 values of -OH clearance rate for P89 and B10 are 49.6μg/mL and 467.3 μg/mL respectively. The EC50 value of -OH clearance rate for PL7 is beyond the minimum limit. The EC50 value of NO2- clearance rate for P89 is 5.3mg/mL; The maxima of NO2- clearance rate for PL7 and B10 are 18.53% and 17.52% respectively when the polysaccharide concentration is above 500μg/mL,【CONCLUSION】The anti-oxidation activity is different between mushrooms and for different free radicals. The tested mushrooms have high ability to clear -OH, middle ability to clear DPPH- but weak ability to clear NO2-. PL7 and B10 have high clearance ability to-OH and DPPH-, but P89 is at the advantage of NO2- clearance ability.