Abstract: Malus hupehensis seedlings were cultured in iron deficiency Sand,then watered the iron-rich apple broth to recover the iron-deficiency phenomenon.The results showed that:T3 treatment (300-fold diluted broth + FeSO4) increased activities of CAT, POD and content of MDA,soluble sugar of Malus hupehensis seedlings significantly.At the same time, the effect of T2 (diluted 100-fold broth + FeSO4) was better than T4 (diluted 500-fold fermentation of Liquid + FeSO4). For three-years iron-deficiency apple, A3 (broth + FeSO4) increased the activities of CAT, POD and content of MDA,soluble sugar.As a whole, T3 treatment (300-fold diluted broth + FeSO4) is the best treatment for both iron-deficiency seeding and three-years iron-deficiency apple.