Abstract:

Abstract：In order to find out some modified methods of ovine sperm capacitation in vitro, the effects of their sperm capacitation, enduring durations and fertilization after respectively adding with 0%、2%、5%、10%、20% heparin at different time (0、30、45、60、90、120min), were compared and analyzed. The results showed that ovine sperm capacitation rate was highest (40.92%), acrosome reaction was lowest(16.59%), enduring durations were longest (18h), the blastula rate was highest (20.63%), when adding with 10μg/ml heparin in the capacitation medium for 45min. The effects of capacitation when adding with caffeine or caffeine and 10μg/ml heparin, were not as good as the effects adding with 10μg/ml heparin alone. It can be recommended that it is applicable for improving ovine capacitation in vitro system with 10μg/ml heparin for 45min, caffeine don’t cooperate with heparin to improve the rate of capacitation.