Abstract:

based on principle and characteristics of the molecular marker techniques of amplified AFLP and ISSR, we have developed a valid method for isolating microsatellites from sugar beet (Beta vulgaris L.) genomic DNA. Fragments flanked by one or two microsatellies were amplified using a microsatellie primer, (AG)10 or (AT)10 etc.. A primer IPl of 27bp designed from the sequenced region at one end of the microsatellie, and for nested PCR another primer IP2 of 21bp based on the sequence between IP1 and the microsatellie were prepared. Then primer IP3 of other side of the microsatellite DNA was designed by nested PCR. The success rate was 21%, which is significantly higher than that of traditional methods. The SSR primers which were got from this study showing higher polymorphic, it is great significance for the following testing of the genetic diversity and genetic linkage map construction.