Abstract:

Using DNA directly extracted, bacterial 16S rDNA PCR amplification, DGGE, cloning and sequencing methods, to study the bacterial diversity in tomato hydroponic culture with different element Ca and P level, indicated interesting results. The results showed that each hydroponic culture of element level had the similar DGGE bands patterns. Compared with the control, every DGGE band patterns of the hydroponic culture changed differently. The bands distribution in the control was more evenly than that of the treatments. Sequencing results showed that the main bacterial divisons in tomato hydroponic culture were Proteobacteria（Alpha，Beta，Gamma and Unclassified），Bacteroidetes and Gram Positive Bacteria.