Abstract:

In order to study the effects of space mutation of wheat, the gliadin and genomic DNA in the SP3 of Baiyingdong 2 were analyzed by acid polyacrylamide gel electrophoresis and SRAP markers, the results showed that the space-flight mutation could induce the variation of gliadin and genomic DNA. 8 mutants of gliadin were screened out from 15 SP3 materials, the induced variation effect also was different in four gliadin protein blocks，among that ω gliadin was the most sensitive and had the greatest variations. The genomic DNA variations of 15 SP3 materials were analyzed using 25 pairs of SRAP primers, and 227 polymorphic bands were amplified, genetic similarity coefficient between SP3 materials and control ranged from 0.821-0.937.