Abstract: In order to know the genetic relationship of progenies from 15 cross combinations got by sexual hybridization (CYG82-114、 CYG No.1、 CYG83-184、CYG82-25、 CYG82-160 and CRG No.3 were selected as male parent ,Baimei sugarcane、CYT 93-159 et al as female parent), 140 progenies were identified by SSR markers, the result showed 109 clones were true hybrids progenies and 27 clones are false progenies, 4 clones belong to self-fertility progenies. According to fathership genetic evaluations, the progenies of 12 cross combinations ( more than 6 clones at least) liked male parent, their band rate account for the band from male parent range from 33.33% -86.67%,and represented low gene heterozygosity, at the same time, they possess relative high genetic rates of male parent.