Abstract: Abstract：The method of low energy ion beam-mediated transformation was adopted to transfer the soybean DNA into two wheat varieties ‘Zhongyu 5’and ‘Huaiyin 9628’.The results suggested that 114 high-protein individuals (protein content >15%) were obtained from T1, and the highest protein content reached 21.44%. The protein content of wheat individuals was greatly increased. The high-protein character could be mostly inherited from T1 to T3, and the character of some lines in T3 was stable basically. It was showed that the method of low energy ion beam-mediated transformation obviously enhanced the transgene and mutagenic effect, and the variant character could be inherited stably. So this method may become to a most efficient way to realize the breakthough of wheat breeding.