Abstract: The standard of seed germination, karyotype, cell culture and suspension culture, active ingredients its introduction and cultivation of Leonurus cardiaca L. and medicinal plants of Leonurus was discussed systematically in the thesis. The resources of medicinal plants of Leonurus was very rich with a variety of chemical active ingredients and pharmacological activity, has broad prospects for application of value. But its tissue culture and cell suspension culture was reported less and its study on improving content of active ingredients using of tissue culture plant need be explorated further and speed up the research. The thesis provided reference for a more rational and effective use of motherwort and medicinal plants of Leonurus.