Abstract:

To study and optimize the extracting technology of the lipopolysaccharide of E.coli. The improved hot phenol method, ultrasonic treatment, boiling were used to extract the lipopolysaccharide from the E.coli cell wall, and the products of three methods were purified and detected the absorption value with 721 spectrophotometer, and then were differently injected the grass carp, test the number of white corpuscles in grass carp blood and catalase activity in different the tissue of grass carp with guaiacol method (data shown with OD value). The lipopolysaccharide extracted with the improved hot phenol, ultrasonic and boiling method at diluted 15-folds was separately detected OD value (1.973, 1.003 and 1.153) and was injected the grass carp. The measured number of white corpuscles was 159 cells/μl, 160 cells/μl and 135 cells/μl, respectively, the catalase activity in blood was 0.09, 0.083 and 0.078, in spleen 0.083, 0.078, and 0.06, respectively. The improved hot phenol method was suitable to extract the high concentrate and immune activity lipopolysaccharide.